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J. T. 'STUBBY' PARKER
CHAIR IN DAIRY FOODS W. James Harper This report is a summary of the activities of Dr. W. James (Jim) Harper as the J. T. "Stubby" Parker Chair in Dairy Foods for the past five years. MAJOR RESEARCH ACCOMPLISHMENTS: Research areas given specific attention include:
Swiss Cheese Research: Ohio is #1 in Swiss Cheese production in the USA and the Parker Chair has a continuing Swiss Cheese Research program since October, 1993. The J.T. Parker Chair, with expenditures of approximately $200,000, primarily funds the program. One M. S. recipient, two Ph.D. recipients and 1 Post Doctoral researcher have completed Swiss Cheese research. Much of the work was accomplished in collaboration with the Brewster Dairy Company, as the company provided most of the cheese used in the research (a minimal amount of cheese was processed in the OSU pilot plant). Major results of this research:
A brief summary of the various research areas are presented in the following table:
Electronic Nose Research: Aroma and taste are among the most important attributes that consumers use in assessing the acceptability of food products. A need exists for objective methods to supplement human sensory evaluation. Specific Instruments were reported as capable of differentiating food aroma and became commercially available four years ago. The Chair predicted these "Electronic Noses" would either become significant tools for food aroma evaluation or "this is one of the largest scams going." The three instruments available at that time differed in respect to sensors, methods for operation and methods for analysis. With no clear indication of which, if any, of the instruments would truly differentiate aromas, negotiation with the three manufacturers led to the purchase of all three pieces at a 50 -66% reduction in cost. The first two years were spent evaluating the electronic noses technology potential. The past two years have assessed the electronic noses value as a research tool and its application to ongoing research projects. Major findings:
Whey Protein Concentrate Research: Whey, long considered a waste product, has been converted to whey proteins and lactose through the application of ultrafiltration. Of these, whey proteins have proven to give the greatest financial return. Whey protein concentrates (WPC) are accepted as an ingredient in a wide range of food products. However, the lack of understanding of factors controlling functionality and the presence of off flavors in WPC have limited their application as food ingredients. Flavor Research: Flavor research on WPC has looked at the identification of volatile compounds associated with different types of WPC, origins of off flavors and methods for improving the flavor of WPC. Particular attention was directed to 34% WPC, which is the major WPC made in the USA, but has received little research attention. Major findings include:
Functionality Research: Functionality research was focussed primarily on 34% WPC, which has received little attention in respect to its functionality and is generally assumed to be much less functional than high protein (>60%) WPC. Major findings of this research include:
Milk Protein Concentrate Research: Milk protein concentrates (MPC) are a new family of milk protein ingredients developed by building on the membrane processing technology successful for making whey protein concentrates. Like many new products, MPCs are not well understood regarding where they are most useful as ingredients or what factors need to be controlled to provide uniform functionality. The work has shown:
Milk Fat Research: The major benefit of milkfat in baked products is the formation of butter flavor during baking, which has never been duplicated. The loss of market share for butter as a bakery ingredient has been its lack of functionality in comparison to vegetable fat products with added emulsifiers. Lipase, in the absence of water and with an excess of glycerol, is able to convert tri-glycerides to mono- and di-glyceride. This research investigated the partial conversion (glycerolysis) of milkfat to mono- and di-glycerides and the application of the modified milk fat as a bakery ingredient.
Effect of Feeding Soybeans on Milk Fat Composition and Flavor: The Parker Chair is a Co-Investigator with Don Palmquist of the Department of Animal Science on a study determining the effect of feeding animals whole soybeans on fatty acid composition and the potential for development of oxidized flavor of milk. Major findings to date include:
Functional Food (Nutriceutical) Research: At the suggestion of the Parker Chair advisory board, attention is now being directed to the investigation of milk components as ingredients in functional (nutriceutical) foods. A paper was prepared regarding the biological properties of whey proteins and the current state of knowledge of their potential role in functional foods. This paper is currently being reviewed by the American Dairy Products Institute for possible publication as a monograph. The possibility that modified beta-lactoglobulin from heated milk may have different physico-chemical and biological properties from normal beta-lactoglobulin is a current investigation. In 1994, other researchers reported finding proteins in the proteose-peptone fraction (proteins remaining soluble after heating to 95 °C and adjustment of the pH to 4.6) of heated milk which interacted with E. coli toxin. These proteins had the amino acid sequence of beta-lactoglobulin and alpha-lactalbumin. The proteins were thought to be lactosylated. Recently, heat treatment was shown by French and New Zealand researchers to cause a Maillard type reaction between beta-lactoglobulin and lactose in milk. An investigation is in progress to determine the characteristics of glycated (lactosylated) beta-lactoglobulin with attention to its functional and biological properties. Previous research showed that an acceptable beverage could be made with tomato juice and yogurt. The potential functional food benefits of this product are being considered. Special attention will be given to potential anti-cancer benefits. Miscellaneous Areas of Research: Cheese Cracker Research: Research was undertaken using a one year industrial grant to determine the reason why it takes so much cheese (20-40% of flour weight) to give a "cheesy" character to cheese crackers. The study investigates ways to provide cheese flavor with less cheese. The four factors found to be associated with the loss of cheese flavor during the manufacture of cheese crackers include: (a) interaction of volatile flavor components with starch and gluten during dough preparation; (b) some masking of cheese flavor by the volatile components in the dough itself; (d) volatilization of volatile compounds and (e) thermal induced changes in the cheese volatiles during baking. The literature reports it necessary to have salt and a pH of less than 5.5 to detect a cheese note in the crackers. Use of different types of cheese at 25% of flour weight resulted in crackers having a "cheesy" note, but gave no indication of the type of cheese used. Attempts to improved cheese flavor through "encapsulation" of cheese with a variety of proteins, starches and gums was not successful, and generally resulted in even less cheesiness in the cracker. Neural Net Research: A commercial program designed for the optimization of manufactured product properties was evaluated for possible use with the Electronic Nose and as a product development tool. The program (ChemCad) was not easily adapted to the electronic nose. However, ChemCad was very useful as a research tool to establish designs for optimization and optimization of process and product formulations. Support of research by other faculty: The chair has provided support to research being conducted by other faculty. Five % release time was provided for the past two years to assist Dr. Zhang's Nattick project on pulsed electric field (PEF) treatment of products to be evaluated for suitability in army rations. The role of the Chair was to provide assistance in the formation of foods designed specifically for PEF. GRADUATE DEGREES COMPLETED: Nine M.S. and five Ph.D. degrees have been completed under the direction of the Chair. Seven additional students are currently working toward completion of degrees. M.S. Kuen Da Jou, M.S. 1994. The effect of added sugars on the thermal behavior of a low lactose whey protein concentrate. Joseph P. Kleinhenz, MS. 1995. Lower molecular weight fatty acids in full fat and low fat Swiss Cheese. Wen Lin Li M. S. 1995. Determination of fatty acids and other volatiles in whey protein concentrates. Tina M. Knisley, M.S. 1995. Comparison of flavor compound detection level by sensory panel versus Aroma Scan. Mahmut Seker M.S. 1996. Bovine/soy milk blends in natural and processed cheese. Poppy Susanto, 1997. The functionality of enzyme modified milkfat in bakery products. Judy Atkinson, M,S. 1997. Assessment of the Sensory Quality of a Medical Nutritional Powder using trained profession panelists and the Alpha MOS Electronic Nose. Cynthia S. Neal, M.S. 1997 The quantitative versus qualitative evaluation of the organoleptic quality of canola oil. Stephen French, M. S. 1997. The effect of milk proteins and emulsifying salts on the characteristics of processed cheese food. Ph.D. Ming-Ju Chen, Ph.D. 1993. Factors affecting consumer acceptance and shelflife of a fermented milk flavored tomato juice product. Stephen Pao, Ph.D. 1994. Halophylic organisms in Sufu, a Chinese cheese. Sungmi Sohn, Ph.D. 1996. Factors affecting the textural properties of low fat Swiss Cheese. Michelle Yang, Ph.D. 1996. The use of cyclodextrins to removed volatile compounds from selected protein products. Kuen Da Jou, Ph.D. 1997. Integrated analysis and pattern recognition of Swiss cheese aroma by SPME/GC/MS and electronic noses. CURRENT GRADUATE STUDENTS Stephen French, Expected Ph.D. 2000. Investigation of Beta-lactoglobulin as a functional food component. Monica De Castro, Expected Ph.D. 1998. Milk protein concentrate research. Sudarat Jianyangyuen, Expected Ph.D. 2000. Research to be determined. Joseph Klenhenz, Expected Ph.D. 2000. Flavor research. Joesphine Kuo, Expected Ph.D. 1999. Milk protein concentrate research. Kwok Man Lee, Expected Ph.D. 1998. 34% whey protein concentrate functionality research. Sarit Seth, Expected M.S. September, 1998. 34% whey protein concentrate flavor research. PRESENTATIONS AND PUBLICATIONS: Since becoming the Parker Chair in Dairy foods, W. J. Harper has given 31 presentations and published 14 technical papers. Presentations: Harper-WJ (1995) Functional properties of whey protein ingredients. IFT Annual Meeting 1995 Book of Abstracts p 45. Kleinhenz-JP; Harper-WJ (1995) Comparison of capillary electrophoresis and capillary gas chromatography analysis of water soluble fatty acids in full fat and low fat Swiss cheese. 1995 IFT annual meeting: book of abstracts, p. 224. Jou-KD; Harper-WJ (1995) The effect of additional disaccharides on thermal behavior of whey protein concentrates determined by DSC. IFT Annual Meeting 1995: book of abstracts p 121. Sohn-S; Harper-WJ (1995) Young's modulus in relation to the textural evaluation of cheese. IFT Annual Meeting 1995:book of abstracts p 123. Sohn-S; Harper-WJ (1995) Comparison of textural characteristics of lowfat and full fat Swiss cheese in relation to microstructure. IFT Annual Meeting 1995: book of abstracts p 123. Tamaro-AC; Harper-WJ; Chism-GW (1995) A rapid method to assess rebodying potential of Swiss-style yogurt gels using a cone and plate viscometer. IFT Annual Meeting 1995: book of abstracts p. 120. Jin-Z; Harper-WJ (1996) Differentiation of enzyme-modified cheese slurries by electronic noses. 1996 IFT annual meeting: book of abstracts, p. 218. Jou-KD; Harper-WJ (1996) Differentiation of seven cheeses using an Electronic Nose. 1996 IFT Annual meeting: Book of abstracts, p. 184. Sungmi-Sohn; Harper-WJ (1996) Modifications of processing procedures to improve the textural properties of low-fat baby Swiss cheese. 1996 IFT annual meeting: book of abstracts, p. 214. Yang-MH; Harper-WJ (1996) Effect of various cyclodextrins on volatile compounds in whey protein concentrate 1996 IFT annual meeting: book of abstracts, p. 151. Yang-MH; Harper-WJ (1995) Effect of beta-cylcodextrin on volatile flavor compounds in whey protein concentrate. IFT Annual Meeting 1995:book of abstracts p 190. De Castro, M. and Harper, W. J. (1997) Basic functional characteristics of milk protein concentrates. IFT Annual Meeting. Book of Abstracts p 227. Harper, W. J. (1997) Seminar presented to the New Zealand Dairy Research Center. The Electronic Nose as a research tool for evaluating food aromas. February 1997. Harper, W. J. (1997) Whey Protein Work Shop. University of Campinas, Campinas, Brazil July 7-11, 1997. Harper, W. J. and Lee, K. M. (1997) Functional Properties and Applications of 34% WPC. Proceedings 1997 International Whey Conference. October 27-29, 1997 International Dairy Federation Bulletin. In Press. Jou, K. D. and Harper, W. J. (1997) Pattern recognition of GC chromatographs of five Swiss cheeses using artificial neural networks and comparison of GC and Electronic Nose Discriminate Plots. IFT Annual Meeting. Book of Abstracts p 225. Lee, K. M. and Harper, W. J. (1997) The function value of 34% whey protein concentrates. IFT Annual Meeting. Book of Abstracts p 226. Sohn, S. and Harper, W. J. (1997) The role of volatile compounds in the flavor of Swiss cheese. J.Dairy Sci. 80:Suppl 1: 133. Susanto, P. and Harper, W. J. (1997) Determination of Mono- and Di-glycerides in milkfat by thin-layer chromatography and densiometric analysis. IFT Annual Meeting. Book of Abstracts p 126. Harper, W. J. (1998) Functional properties of milk protein concentrates. Seminar presented to the New Zealand Dairy Research Center. February 1998. Harper, W. J. (1998) Functional properties of whey protein concentrates. Seminar presented to the New Zealand Dairy Research Institute. February 1998. Harper, W. J. (1998) Factors affecting the functionality of milk proteins in food products. Seminar presented to M&M Mars, Elizabeth Town, PA June 18, 1998. Ruhlman, K. T., Jin, Z. T., Chism, G. W., Harper, W. J. & Zhang, Q. H. (1998) The Development of Cheese Sauce and Salsa to be treated by pulsed electric fields. . IFT Annual Meeting. Book of Abstracts p137. French, S. J. and Harper, W. J. (1998) Effects of milk protein concentrates and emusifier salts on the characteristics of a processed cheese product. IFT Annual Meeting. Book of Abstracts p173. Kuo, C. J. and Harper, W. J. (1998) Effects of dissociating agents on the particle size of milk protein concentrates. IFT Annual Meeting. Book of Abstracts p 205. De Castro, M. and Harper W. J. (1998) Effect of heat treatment on particle size distribution of high protein milk protein concentrates. IFT Annual Meeting. Book of Abstracts p 208. Lee, K. M. and Harper, W. J. (1998) The functionality of 34% whey protein concentrates (WPC) on selected model food systems. IFT Annual Meeting. Book of Abstracts p 211. Huffman, L.H. and Harper, W. J. (1998) Isolating the value of milk through the application of separation technologies. American Dairy Science Annual Meeting. J. Dairy Sci. Supl I. P 73 Harper, W. J. (1998) The electronic nose as a tool in cheese research. Seminar presented to Kraft Foods, Glenview, IL August 21, 1998. Harper, W. J (1998) The of milk proteins on the characteristics of processed cheese. Seminar presented to Kraft Foods, Glenview, IL August 21, 1998. Harper, W. J. (1998) Strengths and Weaknesses of Electronic Noses. Technical Publications: Harper, W. J. (1993) Dairy Substitutes. In Kirk-Othmer Encyclopedia of Chemical Technology. Fourth Edition. Volume No. 7. John Wiley & Sons Inc., N. Y. pp 877-905. Yang, B., Harper, W. J. and Parkin, K. (1993). Control of Lipase-Mediated Glycerolysis Reactions with Butteroil in Dual Liquid Phase Media Devoid of Organic Solvents. J. Agr. Food Chem 41 1905-1909. Yang, B., Harper, W. J. and Parkin, K. (1993) Control of Lipase-Mediated Glycerolysis Reactions with Butteroil in Single liquid phase media with 2-methyl-2-propanol. J. Agr. Food Chem 41:1899-1904. Yang, B., Harper, W. J. and Chen, J. P. (1994). Screening of Commercial Lipases for Production of Mono- and Diacylglycerols from Butter Oil by Enzymatic Glycerolysis. Int. Dairy J. 4:1-13. Jou, K. and Harper, W. J. (1996) Effect of disaccharides on the thermal properties of whey proteins determined by differential scanning calorimetry (DSC). Milchwissenschaft-; 51 (9) 509-512. Jou, K. and Harper, W. J. (1996) Effect of disachharides on the thermal properties of whey proteins determined by various means. Int. J. Dairy Sci. Submitted for Publication. 1997. Mangino, M.E. and Harper, W. J. (1996) Factors affecting application of native and modified proteins in food product. In "Surface Activity of Proteins: Chemical and Physicochemical Modifications. Ed. Shlomo Magdassi. Marcel Dekker, Inc. N.Y. pp 285-322. Harper, W. James, Sohn, Sungmi and Kleinhenz J. (1996) Flavor of Swiss cheese and the Electronic Nose Proceedings Biennial Cheese Symposium August 1996, Utah State University, Logan. Utah. Kleinhenz, J. E. and Harper, W. J. (1997) Comparison of direct capillary electrophoresis and gas capillary chromatographic methods for the determination of lower molecular weight fatty acids in Swiss cheese. Milchwissenschaft 52(4): 200-204. Kleinhenz, J. E. and Harper, W. J. (1997) Lower molecular weight free fatty acids in full fat and low fat Swiss cheese. Milchwissenscaft. 82(11): 622-625. Harper, W. James, Michelle Yang, Sumgmi Sohn (1997) Comparison of three electronic noses for the analysis of aromas of selected dairy products. Proceedings 3rd International Symposium on olfaction and electronic noses. November 4-5, 1996, Miami, Florida. pp 154-164. Harper, W. James, Kuen Da Jou, Sungmi Sohn (1997) The role of fatty acids in the aroma profiles of Swiss cheese as determined by an electronic nose. Proceedings 3rd International Symposium on olfaction and electronic noses. November 4-5, 1996, Miami, Florida. pp 176-185. Yang, M. and Harper, W. J. (1998) Use of solid phase micro extraction of volatile compounds in whey protein concentrates. Milchwissenscaft 53(4): 208-212. Jou, K.D. and Harper, W. J. (1998) Pattern recognition of Swiss cheese aroma compounds by SPME/GC and an electronic nose. Milchwissenschaft 53(5):159-263. TEACHING: The chair has developed and taught four courses in the department:
FST 401, 696 and 650 utilize groups in the learning process and emphasize teamwork, communication skills and critical thinking. As a member of the college's Academy of Teaching and participant in the Kellogg Foundation supported Student Centered Learning Initiative (SCLI), the chair is involved in activities aimed at improving student learning within the college. SCLI includes approximately 50 faculty in the college willing to devote five years toward improving student learning. During Spring quarter of 1998, FST 696 was used as a showcase example of student centered learning. OUTREACH: Primary outreach activities are to provide technical support to food companies in Ohio on request and to support extension activities in dairy foods. Over the past five-years, more than 50 requests for assistance were received. Technical support to the food industry includes:
Copyright © August 1998
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